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Fosmid library production

WebProduction of a beta-carotene through expression of biosynthetic pathway in S. cerevisiae with the carbon source of a xylose: ... Construction of a library with the Golden Gate Assembly method for violacein production in Y. lipolytica: 70.04 mg/L ... Construction of metagenomic fosmid library: Amlakhadi metagenomic library: HPPD: E. coli (Amin ... WebDec 3, 2014 · The CopyControl™ Fosmid Library Production Kits* provide an efficient and improved method for constructing a library of approximately 40 kb clones. The …

The use of fosmid metagenomic libraries in preliminary screening …

WebThe use of culture-independent approaches, such as metagenomics, provides complementary access to environmental microbial diversity. Mangrove environments represent a highly complex system with plenty of opportunities for finding singular functions. In this study we performed a functional screening of fosmid libraries obtained from an oil … WebThe CopyControl™ Fosmid Library Production Kit from Epicentre® was used to construct partial genomic DNA libraries. Concentration of size-selected, end-repaired DNA was determined using Amersham® PicoGreen™ dye and flourimetry. Appropriate quantities of DNA were ligated and packaged according to the manufacturer's protocol. ohp waiver https://gileslenox.com

CopyControl™ Fosmid Library Production Kit with …

WebLucigen also offers the CopyControl Fosmid Library Production Kit. This kit utilizes a strategy of cloning blunt-ended DNA fragments, generated by random shearing of DNA, to produce complete and unbiased genomic libraries. Greater than 106 clones are produced in a single experiment. WebMay 1, 2024 · A soil metagenomic fosmid library was constructed and the clones were screened for antimicrobial function. Our study has shown that heterologous production not only leads to enhanced yield but also provides a platform for further genetic manipulation to increase their efficacy. The peptides were having strong antimicrobial… Show more WebSep 23, 2009 · Fosmid library construction has been divided into four main steps and sub-divided into several parts (see Fig.1 for an overview). Step I (see protocol "DNA extraction from 0.22 μM Sterivex filters" [3]) Part 1: Enzyme-catalyzed cell lysis Part 2: Purification of environmental DNA by CsCl density gradient centrifugation and DNA recovery myid.ihg.com

Construction and screening of a functional metagenomic library …

Category:Construction of Small-Insert and Large-Insert Metagenomic Libraries

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Fosmid library production

Large insert environmental genomic library production

WebExcept genomic DNA, all the materials and protocol as mentioned above were supplied by the Copy Control TM Fosmid Library Production Kit (Epicentre, Madison, WI, United States). Two hundred clones were picked randomly and cultured overnight in 5 ml of LB liquid medium containing 12.5 μg/ml chloramphenicol and 50 μl of auto-induction solution ... WebCopyControl Fosmid Library Production Kits

Fosmid library production

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Fosmids are similar to cosmids but are based on the bacterial F-plasmid. The cloning vector is limited, as a host (usually E. coli) can only contain one fosmid molecule. Fosmids can hold DNA inserts of up to 40 kb in size; often the source of the insert is random genomic DNA. A fosmid library is prepared by extracting the genomic DNA from the target organism and cloning it into the fosmid vector. The ligation mix is then packaged into phage particles and the DNA is transfecte… WebOct 8, 2013 · A fosmid library (average insert size, approximately 40 kb) was constructed via pCC 1FOS™ vector using the CopyControl™ fosmid library production kit then plating on plating Escherichia coli EPI300-T1 R strain, which represented 4×10 8 bp of metagenomic DNA.

WebOverview of the CopyControl Fosmid Library Production Process The CopyControl Fosmid Library Production Kits will produce a complete and unbiased primary fosmid … WebA Fosmid Library is a type of genetic engineering tool used to clone large fragments of DNA. By isolating fragments of DNA, researchers can study the function of genes and their roles in evolution. The library is made up of circular DNA molecules called Fosmids, which contain approximately 40-50 kilobases of DNA, allowing for the study of ...

WebSep 3, 2014 · Product Description. The EpiFOS Fosmid Library Production Kit contains all reagents needed to construct a complete and unbiased fosmid library in about two days … WebAug 16, 2024 · The fosmid library was constructed with the CopyControl™ pCC2FOS™ fosmid library production kit (Epicentre, USA), following manufacturer’s instructions. The library contains about 50,000...

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WebVWR, Part of Avantor - Chemicals and Laboratory Scientific Supplies ohp vision clinicsWebJul 22, 2014 · Three cyanobacterial metagenomic libraries were constructed in the fosmid system. The libraries were screened for various biological activities. Extracts from … ohp triact 70WebThe CopyControl™ Fosmid Library Production Kits* provide an efficient and improved method for constructing a library of approximately 40 kb clones. The CopyControl … ohp single case agreementWebMar 9, 2024 · Sequencing was performed using primers indicated in the CopyControl library production kit for pCC2FOS vector (FWD: 5′‐GTACAACGACACCTAGAC‐`3 ... against the insert‐fosmid end sequences—TAGS of each of the 40 selected fosmids, resulted in 18 contigs successfully tagged to their corresponding fosmid in both ends (5′ and 3′ ends). ... myid.ihg.com employee rateWebMay 11, 2012 · High molecular weight genomic DNA was used to clone the P-SSP7 genome into the pCC1FOS fosmid using the CopyControl Fosmid Library Production kit (Epicentre Biotechnologies) following the manufacturer’s instructions. Briefly, the phage DNA was treated with T4 DNA polymerase and T4 polynucleotide kinase to generate … ohp toothpaste bootsWebSep 23, 2009 · Fosmid library construction has been divided into four main steps and sub-divided into several parts (see Fig.1 for an overview). Step I (see protocol "DNA … ohp vehiclesWebCreative Biogene offers fosmid library construction services for your scientific research as follows: DNA extraction and shearing. Vector construction and infection. Production of a … ohp traffic report