WebProduction of a beta-carotene through expression of biosynthetic pathway in S. cerevisiae with the carbon source of a xylose: ... Construction of a library with the Golden Gate Assembly method for violacein production in Y. lipolytica: 70.04 mg/L ... Construction of metagenomic fosmid library: Amlakhadi metagenomic library: HPPD: E. coli (Amin ... WebDec 3, 2014 · The CopyControl™ Fosmid Library Production Kits* provide an efficient and improved method for constructing a library of approximately 40 kb clones. The …
The use of fosmid metagenomic libraries in preliminary screening …
WebThe use of culture-independent approaches, such as metagenomics, provides complementary access to environmental microbial diversity. Mangrove environments represent a highly complex system with plenty of opportunities for finding singular functions. In this study we performed a functional screening of fosmid libraries obtained from an oil … WebThe CopyControl™ Fosmid Library Production Kit from Epicentre® was used to construct partial genomic DNA libraries. Concentration of size-selected, end-repaired DNA was determined using Amersham® PicoGreen™ dye and flourimetry. Appropriate quantities of DNA were ligated and packaged according to the manufacturer's protocol. ohp waiver
CopyControl™ Fosmid Library Production Kit with …
WebLucigen also offers the CopyControl Fosmid Library Production Kit. This kit utilizes a strategy of cloning blunt-ended DNA fragments, generated by random shearing of DNA, to produce complete and unbiased genomic libraries. Greater than 106 clones are produced in a single experiment. WebMay 1, 2024 · A soil metagenomic fosmid library was constructed and the clones were screened for antimicrobial function. Our study has shown that heterologous production not only leads to enhanced yield but also provides a platform for further genetic manipulation to increase their efficacy. The peptides were having strong antimicrobial… Show more WebSep 23, 2009 · Fosmid library construction has been divided into four main steps and sub-divided into several parts (see Fig.1 for an overview). Step I (see protocol "DNA extraction from 0.22 μM Sterivex filters" [3]) Part 1: Enzyme-catalyzed cell lysis Part 2: Purification of environmental DNA by CsCl density gradient centrifugation and DNA recovery myid.ihg.com