Topflash reporter system

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August undefined, 2024

Web26. jún 2013 · Most assays which are used to screen for Wnt modulating drugs are based on Korinek’s TOPFLASH reporter gene system , where a luciferase enzyme activity is controlled by multiple TCF binding sites (T-cell factor), ... These reporter gene systems open the possibility either to quantify Wnt signaling activation or to image Wnt pathway ... WebThe Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of …

What is the reason for the effect on Renilla signal in the luciferase ...

WebThe Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly ( Photinus pyralis) and Renilla ( Renilla reniformis or sea pansy) … Web21. feb 2012 · TOPflash is a TCF reporter plasmid containing two sets of three copies of wild-type TCF binding sites driven by the thymidine kinase minimal prompter and … teacher airdrops

A diterpenoid derivative 15-oxospiramilactone inhibits Wnt/β …

WebHome - Springer Web( E ) TOPFlash reporter assay in human embryonic kidney (HEK) 293T cells treated with conditioned medium from WT, Bmal1 −/− , Apc +/− , and Apc +/− ; Bmal1 −/− organoids. Luciferase activity from each genotype is shown normalized to WT organoids, and RLU are shown ( n = 3 independent organoid lines per genotype). WebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with 0,... teacher airdrop video reddit

Induction of LEF1 by MYC activates the WNT pathway and …

WebTOPFlash luciferase reporter which has binding sites for. TCF Reporter Plasmid Kit four of transfection grade T cell factor TCF reporter plasmids for over in TOPFlash and FOPFlash wntb-catenin activity assays. -CateninLEF1 transactivates the microRNA-371-373 cluster. Ecm from promega kit from the topflash reporter assay protocol with. WebThe Luciferase Reporter 1000 Assay System (Cat.#. E4550) was designed to meet the needs of users who perform a large number of assays, particularly in 96-well plates. The … teacher airdrop videoWebNational Center for Biotechnology Information teacher ainhoa

"WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF … " - Topflash reporter system

Topflash reporter system

WebPlasmid 8xGTIIC-luciferase from Dr. Stefano Piccolo's lab contains the insert synthetic TEAD luciferase reporter and is published in Nature. 2011 Jun 8;474(7350):179-83. doi: 10.1038/nature10137. This plasmid is available through Addgene. WebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. …

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WebThe first transcription-based luciferase reporter of Wnt/β-catenin signaling, TOPFlash, was designed by Korinek et al. ( 4 ). The TOPFlash reporter contains three TCF response … WebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold …

WebThe Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 –20 moles of luciferase can be … WebLuciferase reporter assays allow you to investigate promoter activity by measuring light output from luciferase enzyme that is expressed under the control of your promoter of interest. Luciferase genes vary in stability, light output and spectral characteristics, and reporter kits are available with different luciferases for both single- and dual-spectral …

WebAberrant activation of the Wnt/β-catenin signaling pathway is closely related to tumorigenesis. In order to identify potent small molecules to treat the over-activated Wnt signaling-mediated cancer, such as colon cancer, we established a mammalian cell line-based reporter gene screening system. WebThis is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated. There are 6 mutated TCF/LEF …

WebThe TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / β-catenin signaling pathway in the cultured cells. The kit contains transfection-ready TCF/LEF luciferase …

WebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin). The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites. teacher aim teacher airdrop memeWeb15. máj 2024 · We designed several TopFlash constructs carrying various domains of β-catenin, particularly Wild-type (WT), S33A, ΔNTAD, ΔCTAD, ΔNTAD/ΔCTAD and ΔARM (Supplementary Fig. 5 ). The latter three... teacher airfare discountWebModified/Novel Luciferases. AkaLuc: A modified firefly luciferase engineered to use the red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared range, enabling enhanced bioluminescence imaging in vivo.; Luciferases with tunable wavelengths: NanoLuc® with either SNAP‐tag or HaloTag7 tag in which labeling … teacher aimsWeb22. aug 2024 · Insights into the underlying mechanisms of competitive endogenous RNAs (ceRNAs) were determined by bioinformatics analysis, dual-luciferase reporter assays, … teacher airpod caseWebTopflash Dual Light Reporter Gene Assay System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … teacher akoWebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is … teacher airport parking discount